8.5. Extracting root sum of squares from edge-wise timeseries.

This example uses a RSSETSMarker to compute root sum of squares of the edge-wise timeseries using the Schaefer parcellation (100 rois and 200 rois, 17 Yeo networks) for a 4D nifti BOLD file.

Authors: Leonard Sasse, Sami Hamdan, Nicolas Nieto, Synchon Mandal

License: BSD 3 clause

import tempfile

import junifer.testing.registry  # noqa: F401
from junifer.api import collect, run
from junifer.storage import SQLiteFeatureStorage
from junifer.utils import configure_logging

Set the logging level to info to see extra information:

configure_logging(level="INFO")
/__w/junifer/junifer/junifer/utils/logging.py:155: UserWarning: The '__version__' attribute is deprecated and will be removed in MarkupSafe 3.1. Use feature detection, or `importlib.metadata.version("markupsafe")`, instead.
  vstring = str(getattr(module, "__version__", None))
2025-09-22 15:48:15,700 - JUNIFER - INFO - ===== Lib Versions =====
2025-09-22 15:48:15,700 - JUNIFER - INFO - click: 8.1.8
2025-09-22 15:48:15,700 - JUNIFER - INFO - numpy: 1.26.4
2025-09-22 15:48:15,700 - JUNIFER - INFO - scipy: 1.15.0
2025-09-22 15:48:15,701 - JUNIFER - INFO - datalad: 1.1.6
2025-09-22 15:48:15,701 - JUNIFER - INFO - pandas: 2.1.4
2025-09-22 15:48:15,701 - JUNIFER - INFO - nibabel: 5.3.2
2025-09-22 15:48:15,701 - JUNIFER - INFO - nilearn: 0.10.4
2025-09-22 15:48:15,701 - JUNIFER - INFO - sqlalchemy: 2.0.43
2025-09-22 15:48:15,701 - JUNIFER - INFO - ruamel.yaml: 0.18.15
2025-09-22 15:48:15,701 - JUNIFER - INFO - tqdm: 4.66.6
2025-09-22 15:48:15,701 - JUNIFER - INFO - templateflow: 24.2.2
2025-09-22 15:48:15,701 - JUNIFER - INFO - junifer_data: None
2025-09-22 15:48:15,701 - JUNIFER - INFO - junifer: 0.0.7.dev206
2025-09-22 15:48:15,701 - JUNIFER - INFO - ========================

Define the DataGrabber interface

datagrabber = {
    "kind": "SPMAuditoryTestingDataGrabber",
}

Define the markers interface

markers = [
    {
        "name": "Schaefer100x17_RSSETS",
        "kind": "RSSETSMarker",
        "parcellation": "Schaefer100x17",
    },
    {
        "name": "Schaefer200x17_RSSETS",
        "kind": "RSSETSMarker",
        "parcellation": "Schaefer200x17",
    },
]

Create a temporary directory for junifer feature extraction: At the end you can read the extracted data into a pandas.DataFrame.

with tempfile.TemporaryDirectory() as tmpdir:
    # Define the storage interface
    storage = {
        "kind": "SQLiteFeatureStorage",
        "uri": f"{tmpdir}/test.sqlite",
    }
    # Run the defined junifer feature extraction pipeline
    run(
        workdir=tmpdir,
        datagrabber=datagrabber,
        markers=markers,
        storage=storage,
        elements=["sub001"],  # we calculate for one subject only
    )
    # Collect extracted features data
    collect(storage=storage)
    # Create storage object to read in extracted features
    db = SQLiteFeatureStorage(uri=storage["uri"])

    # List all the features
    print(db.list_features())
    # Read extracted features
    df_rssets = db.read_df(feature_name="BOLD_Schaefer200x17_RSSETS_rss_ets")
2025-09-22 15:48:15,703 - JUNIFER - INFO - Registering RSSETSMarker in marker
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating Marker Collection
2025-09-22 15:48:15,703 - JUNIFER - INFO - DataGrabber output type: ['BOLD', 'T1w']
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating Data Reader:
2025-09-22 15:48:15,703 - JUNIFER - INFO - Data Reader output type: ['BOLD', 'T1w']
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating Marker: Schaefer100x17_RSSETS
2025-09-22 15:48:15,703 - JUNIFER - INFO - Marker output type: ['timeseries']
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating storage for Schaefer100x17_RSSETS
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating Marker: Schaefer200x17_RSSETS
2025-09-22 15:48:15,703 - JUNIFER - INFO - Marker output type: ['timeseries']
2025-09-22 15:48:15,703 - JUNIFER - INFO - Validating storage for Schaefer200x17_RSSETS
2025-09-22 15:48:15,703 - JUNIFER - INFO - Getting element sub001
2025-09-22 15:48:17,771 - JUNIFER - INFO - Fitting pipeline
2025-09-22 15:48:17,771 - JUNIFER - INFO - Reading BOLD from /tmp/tmpqdjk2gsb/sub001_bold.nii.gz
2025-09-22 15:48:17,771 - JUNIFER - INFO - BOLD is of type NIFTI
2025-09-22 15:48:17,772 - JUNIFER - INFO - Reading T1w from /tmp/tmpqdjk2gsb/sub001_T1w.nii.gz
2025-09-22 15:48:17,772 - JUNIFER - INFO - T1w is of type NIFTI
2025-09-22 15:48:17,773 - JUNIFER - INFO - Fitting marker Schaefer100x17_RSSETS
2025-09-22 15:48:17,773 - JUNIFER - INFO - Computing BOLD
2025-09-22 15:48:17,773 - JUNIFER - INFO - Parcellation will be warped from MNI152NLin6Asym to MNI152Lin using highest resolution
2025-09-22 15:48:17,773 - JUNIFER - INFO - Parcellation parameters:
2025-09-22 15:48:17,773 - JUNIFER - INFO -      resolution: None
2025-09-22 15:48:17,773 - JUNIFER - INFO -      n_rois: 100
2025-09-22 15:48:17,773 - JUNIFER - INFO -      yeo_networks: 17
2025-09-22 15:48:17,773 - JUNIFER - INFO - Resolution set to None, using highest resolution.
2025-09-22 15:48:17,849 - JUNIFER - INFO - Loading parcellation: /github/home/junifer_data/v4/parcellations/Schaefer2018/Yeo2011/Schaefer2018_100Parcels_17Networks_order_FSLMNI152_1mm.nii.gz
2025-09-22 15:48:20,149 - JUNIFER - INFO - Downloading template MNI152Lin (T1w in resolution 1)
2025-09-22 15:48:20,654 - JUNIFER - INFO - antsApplyTransforms command to be executed:
antsApplyTransforms -d 3 -e 3 -n 'GenericLabel[NearestNeighbor]' -i /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer100x17_from_MNI152NLin6Asym_to_MNI152Lin_8ef30dd4-97cb-11f0-a4c0-5ace1b2a80e5upnq24_w/prewarp_parcellation.nii.gz -r /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer100x17_from_MNI152NLin6Asym_to_MNI152Lin_8ef30dd4-97cb-11f0-a4c0-5ace1b2a80e5upnq24_w/MNI152Lin_T1w.nii.gz -t /github/home/junifer_data/v4/.git/annex/objects/JQ/Pp/SHA256E-s145422752--5091350b36f951d455dabd429ebe86c493c75a0217dca311ae355f1d62e080b0.h5/SHA256E-s145422752--5091350b36f951d455dabd429ebe86c493c75a0217dca311ae355f1d62e080b0.h5 -o /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer100x17_from_MNI152NLin6Asym_to_MNI152Lin_8ef30dd4-97cb-11f0-a4c0-5ace1b2a80e5upnq24_w/parcellation_warped.nii.gz
2025-09-22 15:48:26,963 - JUNIFER - INFO - antsApplyTransforms command succeeded with the following output:

2025-09-22 15:48:30,010 - JUNIFER - INFO - Storing in <SQLiteFeatureStorage @ /tmp/tmpfmfi45t0/test.sqlite (multiple output)>
2025-09-22 15:48:30,028 - JUNIFER - INFO - Fitting marker Schaefer200x17_RSSETS
2025-09-22 15:48:30,028 - JUNIFER - INFO - Computing BOLD
2025-09-22 15:48:30,028 - JUNIFER - INFO - Parcellation will be warped from MNI152NLin6Asym to MNI152Lin using highest resolution
2025-09-22 15:48:30,028 - JUNIFER - INFO - Parcellation parameters:
2025-09-22 15:48:30,029 - JUNIFER - INFO -      resolution: None
2025-09-22 15:48:30,029 - JUNIFER - INFO -      n_rois: 200
2025-09-22 15:48:30,029 - JUNIFER - INFO -      yeo_networks: 17
2025-09-22 15:48:30,029 - JUNIFER - INFO - Resolution set to None, using highest resolution.
2025-09-22 15:48:30,103 - JUNIFER - INFO - Loading parcellation: /github/home/junifer_data/v4/parcellations/Schaefer2018/Yeo2011/Schaefer2018_200Parcels_17Networks_order_FSLMNI152_1mm.nii.gz
2025-09-22 15:48:32,400 - JUNIFER - INFO - Downloading template MNI152Lin (T1w in resolution 1)
2025-09-22 15:48:32,905 - JUNIFER - INFO - antsApplyTransforms command to be executed:
antsApplyTransforms -d 3 -e 3 -n 'GenericLabel[NearestNeighbor]' -i /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer200x17_from_MNI152NLin6Asym_to_MNI152Lin_964170da-97cb-11f0-a4c0-5ace1b2a80e54izceu00/prewarp_parcellation.nii.gz -r /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer200x17_from_MNI152NLin6Asym_to_MNI152Lin_964170da-97cb-11f0-a4c0-5ace1b2a80e54izceu00/MNI152Lin_T1w.nii.gz -t /github/home/junifer_data/v4/.git/annex/objects/JQ/Pp/SHA256E-s145422752--5091350b36f951d455dabd429ebe86c493c75a0217dca311ae355f1d62e080b0.h5/SHA256E-s145422752--5091350b36f951d455dabd429ebe86c493c75a0217dca311ae355f1d62e080b0.h5 -o /tmp/junifer/tmpsdoq4hv3/ants_parcellation_warper_Schaefer200x17_from_MNI152NLin6Asym_to_MNI152Lin_964170da-97cb-11f0-a4c0-5ace1b2a80e54izceu00/parcellation_warped.nii.gz
2025-09-22 15:48:44,230 - JUNIFER - INFO - antsApplyTransforms command succeeded with the following output:

2025-09-22 15:48:46,735 - JUNIFER - INFO - Storing in <SQLiteFeatureStorage @ /tmp/tmpfmfi45t0/test.sqlite (multiple output)>
2025-09-22 15:48:46,758 - JUNIFER - INFO - Marker collection fitting done
2025-09-22 15:48:46,768 - JUNIFER - INFO - Collecting data using SQLiteFeatureStorage
2025-09-22 15:48:46,768 - JUNIFER - INFO - Collecting data from /tmp/tmpfmfi45t0/*test.sqlite

file: 0it [00:00, ?it/s]

feature:   0%|          | 0/2 [00:00<?, ?it/s]
feature: 100%|██████████| 2/2 [00:00<00:00, 73.33it/s]

file: 1it [00:00, 24.99it/s]
2025-09-22 15:48:46,809 - JUNIFER - INFO - Collect done
{'de47d0513cf22df792d264ecc363085a': {'datagrabber': {'class': 'SPMAuditoryTestingDataGrabber', 'types': ['BOLD', 'T1w']}, 'dependencies': {'nilearn': '0.10.4'}, 'datareader': {'class': 'DefaultDataReader'}, 'type': 'BOLD', 'marker': {'agg_method': 'mean', 'agg_method_params': None, 'class': 'RSSETSMarker', 'masks': None, 'name': 'Schaefer100x17_RSSETS_rss_ets', 'parcellation': 'Schaefer100x17'}, '_element_keys': ['subject'], 'name': 'BOLD_Schaefer100x17_RSSETS_rss_ets'}, '0ff7a9e8f65fc25885bd0cd4936f87a4': {'datagrabber': {'class': 'SPMAuditoryTestingDataGrabber', 'types': ['BOLD', 'T1w']}, 'dependencies': {'nilearn': '0.10.4'}, 'datareader': {'class': 'DefaultDataReader'}, 'type': 'BOLD', 'marker': {'agg_method': 'mean', 'agg_method_params': None, 'class': 'RSSETSMarker', 'masks': None, 'name': 'Schaefer200x17_RSSETS_rss_ets', 'parcellation': 'Schaefer200x17'}, '_element_keys': ['subject'], 'name': 'BOLD_Schaefer200x17_RSSETS_rss_ets'}}

Now we take a look at the dataframe

root_sum_of_squares_ets
subject timepoint
sub001 0 2502.888057
1 168.174038
2 102.849715
3 132.639660
4 109.615993


Total running time of the script: (0 minutes 31.129 seconds)

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